Thin-layer chromatography (TLC) is a technique of chromatography for the separation of a mixture by using a thin layer of stationary phase supported on the insert plate.
Benzene-Methanol, Alcohol, Silica Gel (Chromatographic grade)
Beakers, Pipette, Slides, Chromatographic tank, Silica Gel TLC Plates, iodine Chamber (place iodine crystals gas jar and cover)
- Take a silica gel coated glass plate.
- Draw baseline about 2 cm above the base of the plate with thin pencil.
- Placemarks of given lipid sample by using a capillary tube on the baseline.
- Place the plate in a chromatographic tank containing benzene-methanol (80:20) mixture used as the solvent.
- Wait for 45 minutes to 1 hour for the solvent to rise and to separate different fractions of lipid.
- Remove the plate and place in the gas jar containing iodine vapours produced by the sublimation of iodine from Its crystals for 3 to 5 minutes.
- Spots of different colors of the components of lipids will be seen on the plate.