Detection of presence of different lipid components in an oil sample by TLC

Principle.

Thin-layer chromatography (TLC) is a technique of chromatography for the separation of a mixture by using a thin layer of stationary phase supported on the insert plate.

Chemical Required.

Benzene-Methanol, Alcohol, Silica Gel (Chromatographic grade)

Apparatus.

Beakers, Pipette, Slides, Chromatographic tank, Silica Gel TLC Plates, iodine Chamber (place iodine crystals gas jar and cover)

Procedure.

  1. Take a silica gel coated glass plate.
  2. Draw baseline about 2 cm above the base of the plate with thin pencil.
  3. Placemarks of given lipid sample by using a capillary tube on the baseline.
  4. Place the plate in a chromatographic tank containing benzene-methanol (80:20) mixture used as the solvent.
  5. Wait for 45 minutes to 1 hour for the solvent to rise and to separate different fractions of lipid.
  6. Remove the plate and place in the gas jar containing iodine vapours produced by the sublimation of iodine from Its crystals for 3 to 5 minutes.
  7. Spots of different colors of the components of lipids will be seen on the plate.

Result.

The oil components are separated.
and can be seen on TLC plate.
Detection of presence of different lipid components in an oil sample by TLC
Detection of presence of different lipid components in an oil sample by TLC Detection of presence of different lipid components in an oil sample by TLC Reviewed by Genuine Chemistry on March 03, 2020 Rating: 5

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